Prnp Polyclonal Antibody for Mouse(Mus musculus) | EIAab.comPrnp Polyclonal Antibody for Mouse(Mus musculus)

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Prnp (Gene name),

Major prion protein (Protein name ), PRIO_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

Prnp(Prn-p;Prp);

Protein name:

Major prion protein(PrP);

Alternative:

PrP33-35C;PrP27-30;

CD:

CD230;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Monomer and homodimer. Has a tendency to aggregate into amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Soluble oligomers may represent an intermediate stage on the path to fibril formation. Copper binding may promote oligomerization. Interacts with GRB2, APP, ERI3/PRNPIP and SYN1. Mislocalized cytosolically exposed PrP interacts with MGRN1; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement (By similarity). Interacts with APP.

Function:

The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).

Subcellular Location:

Cell membrane Lipid-anchor GPI-anchor Golgi apparatus

Protein Attributes:

Sequence length:

254

Sequence:

50:

100:

150:

200:

250:

254:

LIVG

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

mmu:19122

SMR:

P04925

String:

P04925

Pfam:

PF00377

UniGene:

Mm.648

Uniprot:

P04925

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ELISA Kit for Mouse PrP

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E9345h

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96T

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ELISA Kit for Mouse PrP

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E9345m

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96T

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ELISA Kit for Mouse PrP

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E9345c

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ELISA Kit for Mouse PrP

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ELISA Kit for Mouse PrP

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E9345r

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ELISA Kit for Mouse PrP

Cat.:

E9345b

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ELISA Kit for Mouse PrP

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ELISA Kit for Mouse PrP

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E9345p

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96T

CLIA Kit for Mouse PrP

Cat.:

U9345h

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96T

CLIA Kit for Mouse PrP

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U9345r

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96T

CLIA Kit for Mouse PrP

Cat.:

U9345m

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96T

CLIA Kit for Mouse PrP

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U9345b

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96T

CLIA Kit for Mouse PrP

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CLIA Kit for Mouse PrP

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CLIA Kit for Mouse PrP

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CLIA Kit for Mouse PrP

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U9345p

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96T

Polyclonal Antibody for Mouse PrP

Cat.:

P9345Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse PrP

Cat.:

P9345Rb-m

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40ug/0.2ml

Monoclonal Antibody for Mouse PrP

Protein for Mouse PrP

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-106
Plasma	98	92-108

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-102%	92-103%	95-105%	92-107%
EDTA plasma(n=5)	88-96%	93-101%	94-102%	95-106%
heparin plasma(n=5)	93-104%	94-105%	101-106%	95-109%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Distinct prion proteins in short and long scrapie incubation period mice."
Westaway D. , Goodman P.A. , Mirenda C.A. , McKinley M.P. , Carlson G.A. , Prusiner S.B.
Cell51:651-662(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: I/LnJ.
strain: NZW.
2.

"Molecular cloning and complete sequence of prion protein cDNA from mouse brain infected with the scrapie agent."
Locht C. , Chesebro B. , Race R. , Keith J.M.
Proc. Natl. Acad. Sci. U.S.A.83:6372-6376(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Molecular pathology of scrapie-associated fibril protein (PrP) in mouse brain affected by the ME7 strain of scrapie."
Hope J. , Multhaup G. , Reekie L.J.D. , Kimberlin R.H. , Beyreuther K.
Eur. J. Biochem.172:271-277(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE
4.

"Complete genomic sequence and analysis of the prion protein gene region from three mammalian species."
Lee I.Y. , Westaway D. , Smit A.F.A. , Wang K. , Seto J. , Chen L. , Acharya C. , Ankener M. , Baskin D. , Cooper C. , Yao H. , Prusiner S.B. , Hood L.E.
Genome Res.8:1022-1037(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: NZW.
tissue: Brain.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
6.

"Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain."
Chesebro B. , Race R. , Wehrly K. , Nishio J. , Bloom M. , Lechner D. , Bergstrom S. , Robbins K. , Mayer L. , Keith J.M. , Garon C. , Haase A.
Nature315:331-333(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-164
7.

"Prion protein is necessary for normal synaptic function."
Collinge J. , Whittington M.A. , Sidle K.C. , Smith C.J. , Palmer M.S. , Clarke A.R. , Jefferys J.G.
Nature370:295-297(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE
8.

"Mice deficient for prion protein exhibit normal neuronal excitability and synaptic transmission in the hippocampus."
Lledo P.M. , Tremblay P. , DeArmond S.J. , Prusiner S.B. , Nicoll R.A.
Proc. Natl. Acad. Sci. U.S.A.93:2403-2407(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE
9.

"Copper stimulates endocytosis of the prion protein."
Pauly P.C. , Harris D.A.
J. Biol. Chem.273:33107-33110(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
10.

"Post-translational hydroxylation at the N-terminus of the prion protein reveals presence of PPII structure in vivo."
Gill A.C. , Ritchie M.A. , Hunt L.G. , Steane S.E. , Davies K.G. , Bocking S.P. , Rhie A.G.O. , Bennett A.D. , Hope J.
EMBO J.19:5324-5331(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: HYDROXYLATION AT PRO-44
11.

"PrPC directly interacts with proteins involved in signaling pathways."
Spielhaupter C. , Schaetzl H.M.
J. Biol. Chem.276:44604-44612(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH GRB2; ERI3 AND SYN1;MUTAGENESIS OF PRO-101 AND PRO-104
12.

"Prion, amyloid beta-derived Cu(II) ions, or free Zn(II) ions support S-nitroso-dependent autocleavage of glypican-1 heparan sulfate."
Mani K. , Cheng F. , Havsmark B. , Jonsson M. , Belting M. , Fransson L.A.
J. Biol. Chem.278:38956-38965(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: COPPER BINDING;SUBCELLULAR LOCATION;FUNCTION
13.

"Copper-dependent co-internalization of the prion protein and glypican-1."
Cheng F. , Lindqvist J. , Haigh C.L. , Brown D.R. , Mani K.
J. Neurochem.98:1445-1457(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;COPPER-BINDING
14.

"Prion protein (PrPc) positively regulates neural precursor proliferation during developmental and adult mammalian neurogenesis."
Steele A.D. , Emsley J.G. , Ozdinler P.H. , Lindquist S. , Macklis J.D.
Proc. Natl. Acad. Sci. U.S.A.103:3416-3421(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;GLYCOSYLATION;FUNCTION;TISSUE SPECIFICITY
15.

"Functional depletion of mahogunin by cytosolically exposed prion protein contributes to neurodegeneration."
Chakrabarti O. , Hegde R.S.
Cell137:1136-1147(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MGRN1
16.

"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B. , Bausch-Fluck D. , Henderson C. , O'Brien R. , Bibel M. , Schiess R. , Aebersold R. , Watts J.D.
Nat. Biotechnol.27:378-386(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-196
17.

"Cellular prion protein mediates impairment of synaptic plasticity by amyloid-beta oligomers."
Lauren J. , Gimbel D.A. , Nygaard H.B. , Gilbert J.W. , Strittmatter S.M.
Nature457:1128-1132(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;INTERACTION WITH APP;FUNCTION
18.

"Prion protein (PrP) knock-out mice show altered iron metabolism: a functional role for PrP in iron uptake and transport."
Singh A. , Kong Q. , Luo X. , Petersen R.B. , Meyerson H. , Singh N.
PLoS ONE4:E6115-E6115(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;FUNCTION;GLYCOSYLATION;TISSUE SPECIFICITY
19.

"Early onset prion disease from octarepeat expansion correlates with copper or zinc binding properties."
Stevens D.J. , Walter E.D. , Rodriguez A. , Draper D. , Davies P. , Brown D.R. , Millhauser G.L.
PLoS Pathog.5:E1000390-E1000390(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: COPPER-BINDING
20.

"Axonal prion protein is required for peripheral myelin maintenance."
Bremer J. , Baumann F. , Tiberi C. , Wessig C. , Fischer H. , Schwarz P. , Steele A.D. , Toyka K.V. , Nave K.A. , Weis J. , Aguzzi A.
Nat. Neurosci.13:310-318(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE
21.

"NMR structure of the mouse prion protein domain PrP(121-321)."
Riek R. , Hornemann S. , Wider G. , Bileter M. , Glockshuber R. , Wuethrich K.
Nature382:180-182(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 120-230
22.

"NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231)."
Riek R. , Hornemann S. , Wider G. , Glockshuber R. , Wuethrich K.
FEBS Lett.413:282-288(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 23-231

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